Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC MOPS | 1132-61-2 | 99.95% | 10 G
MOPS is a buffering agent used in biological applications. It functions to maintain the pH of mammalian cell culture media. Additionally, it has been shown to significantly enhance the thermal stability of BSA.
- Functions as a buffering agent in biology.
- Maintains the pH of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3894476 TRIS USP MULTICOM 50KG FLOWMOR
Zymo Research Corporation RBC Lysis Buffer, 50 ml
RBC Lysis Buffer (50 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
GENERAL LABORATORY PRODUCTS INC SALINE .85 TW80 PC20X125 100CS
NC3313467 SALINE .85 TW80 PC20X125 100CS
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 100ml
HEPES buffer solution 1 M in H2O | Mol Wt: | 7365-45-9 | MFCD00006158 | 100ml
Sigma Aldrich Fine Chemicals Biosciences HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
Gold Biotechnology Inc HEPES Sodium Salt 1 kg
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups such as Tris This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers This buffer is capable of forming radicals and is therefore not suitable for redox reactions It is suitable for use in the bicinchoninic acid (BCA) assay
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(t-Bu) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 100 MG
NODAGA-tris(t-Bu ester) is a tri-tert-butyl ester-protected NODAGA chelator intended for conjugation to biomolecules and subsequent radiolabeling for positron emission tomography (PET) studies. It provides a protected, bifunctional scaffold that facilitates attachment to peptides, proteins, and antibodies and enables stable chelation of radiometals after deprotection.
- Protected NODAGA chelator for conjugation to biomolecules.
- Suitable for labeling peptides, proteins, and antibodies.
- Designed for radiolabeling workflows for PET imaging.
- High purity (99.8%) with RP-HPLC documentation available.
- Available as solid and as DMSO solutions for convenience.
- Molecular formula C27H49N3O8; molecular weight 543.69 g/mol.
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G