Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova MES Elution Buffer, pH 6.5. 1000mL, Sterile
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
STEMCELL Technologies EasySep™ Red Blood Cell Lysis Buffer, Size: 100 mL
EasySep™ Red Blood Cell Lysis Buffer is for use as directed with EasySep™ or RoboSep™ Whole Blood kits for the lysis of red blood cells prior to separation from whole blood.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 1 L
Synonym: TBS, 10 ×, solution. Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 25 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Abcam 10 x RIPA Buffer, 15ML
10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline | MFCD00131855 | 1L
Phosphate Buffered Saline | MFCD00131855 | 1L
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
Mitegen HEPES/ NaOH, 1M, pH7.5, 250 ml, CAS # 7365-45-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Zymo Research Corporation ZymoPURE Binding Buffer, 210 ml
ZymoPURE Binding Buffer is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
![Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-D22150-1000.0.JPG-250.jpg)
Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
Ready to use sterile 1x solution
Contains: 137 mM Sodium Chloride
2.7 mM Potassium Chloride
10 mM Phosphates
Does not contain Calcium or Magnesium
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Sigma Aldrich Fine Chemicals Biosciences 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | 90217-02-0 | MFCD00467521 | 10MG
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | Purity: >=85% (HPLC) | Mol Wt: 311.77 | 90217-02-0 | MFCD00467521 | 10MG