Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Axxora LLC Hepes Buffer 1 M pH 7.5
Hepes Buffer is one of the best general purpose buffers available for biological research. It has been used to advantage in tissue culture, oxidative phosphorylation and protein synthesis with cell-free bacterial systems. Hepes is a recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 100g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 100g
Teknova 1M Tris-HCl, pH 6.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.6) - 1L
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a physiological pH range, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 10X concentration, and can be diluted to support its application.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
DULBECCOS Dulbeccos Modified Eagles500ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline1L
PHOSPHATE Phosphate buffered saline1L
Sigma Aldrich Fine Chemicals Biosciences Iscoves Modified Dulbeccos
ISCOVES Iscoves Modified Dulbeccos